Potentiometric enzyme channelling immunosensor for proteins
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چکیده
منابع مشابه
Potentiometric enzyme channeling immunosensor for proteins.
A potentiometric immunosensor for the detection of human IgG has been developed using an asymmetric, ion-selective membrane with immobilized adenosine deaminase and IgG. A protein A-alkaline phosphatase conjugate binds to the immobilized IgG, creating a bienzymatic catalytic layer. In the presence of sample IgG, the conjugate does not bind to the membrane. Instead, the intermediate in the two-s...
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3 IN VIVO IS NOT THE SAME AS IN VITRO 4 ORGANIZATION LEADS TO CHANNELLING 9 STATIC VERSUS DYNAMIC CHANNELS 10 SOME CONTROVERSIES ABOUT DYNAMIC CHANNELLING 12 MODELLING STRATEGIES FOR STUDYING ENZYMOLOGY IN VIVO 18 CONCLUDING REMARKS 23
متن کاملMetabolic channelling of carbamoyl phosphate in the hyperthermophilic archaeon Pyrococcus furiosus: dynamic enzyme-enzyme interactions involved in the formation of the channelling complex.
Protection of thermolabile metabolites and coenzymes is a somewhat neglected but essential aspect of the molecular physiology of hyperthermophiles. Detailed information about the mechanisms used by thermophiles to protect these thermolabile metabolites and coenzymes is still scarce. A case in point is CP (carbamoyl phosphate), a precursor of pyrimidines and arginine, which is an extremely labil...
متن کاملPotentiometric studies of native and flavin-substituted Old Yellow Enzyme.
We have measured the redox potentials for the flavin cofactor of native Old Yellow Enzyme and for a series of chemically modified flavin derivatives bound to the apoprotein. These flavin derivatives have midpoint potentials ranging from -120 to -300 mV in free solution. For the native enzyme, the midpoint potential of the first one-electron couple EFMNox + e- in equilibrium EFMN-. is E1 = -245 ...
متن کاملChannelling Evolution
Individuals within a wild population show remarkably little morphological variation, given the amount of environmental variation they encounter during development and the amount of genetic variation within the population. This phenotypic constancy led to the proposal that individuals were somehow buffered, or canalized, against genetic and environmental variation (Waddington 1942). Clearly, suc...
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ژورنال
عنوان ژورنال: Biosensors and Bioelectronics
سال: 1991
ISSN: 0956-5663
DOI: 10.1016/0956-5663(91)80027-u